Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Relationship between single-nucleotide polymorphisms in un-translated region of human leukocyte antigen-G and preeclampsia

Zhang Zhan¹ , Zhang Nan¹, Zhang Linlin², Jia Liting², Li Hong¹

¹Department of Obstetrics and Gynecology; ²Department of Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.

For correspondence:- Zhang Zhan Email: zhanzhang_zz@163.com Tel:+8613837362662

Received: 25 January 2017 Accepted: 11 June 2017 Published: 31 July 2017

Citation: Zhan Z, Nan Z, Linlin Z, Liting J, Hong L. Relationship between single-nucleotide polymorphisms in un-translated region of human leukocyte antigen-G and preeclampsia. Trop J Pharm Res 2017; 16(7):1621-1629 doi: 10.4314/tjpr.v16i7.22

© 2017 The authors.
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Abstract

Purpose: The aim of this study was to unravel the link between human leukocyte antigen-G un-translated region (HLA-G 3-UTR) single-nucleotide polymorphism (SNP) and preeclampsia by examining polymorphisms in HLA-G 3-UTR in preeclampsia patients and their newborns, as well as those of women with normal pregnancy and their newborns.

Methods: Single pregnant mothers and their newborns at the Third Affiliated Hospital of Zhengzhou University, China, were divided into preeclampsia group (144 cases), and normal pregnancy group (122 cases). Blood samples from the two groups were collected for DNA extraction, and the DNA samples were analyzed for HLA-G 3'-UTR SNP by polymerase chain reaction (RT-PCR) and direct sequencing method.

Results: The frequency of CG genotype in HLA-G 3-UTR 3127 gene in the preeclampsia group was significantly lower, while the frequency of AA genotype in HLA-G 3-UTR 3172 gene in the preeclampsia group was significantly higher (p < 0.05) when compared with the normal pregnancy group. Furthermore, frequencies of the compatible genotype AA/AA (mothers/newborns) in HLA-G 3-UTR 3172 gene in the preeclampsia group were significantly higher relative to the normal pregnancy group (p< 0.05).

Conclusion: These results suggest that when CC/GG is the compatible genotype in HLA-G 3-UTR 3127 gene of mothers/newborns, preeclampsia risk may decrease, but it may increase if the compatible genotype is AA/AA. Thus, SNP in HLA-G 3-UTR 3127 gene may be a protective factor against preeclampsia in Chinese Han pregnant women, while SNP in 3172 gene may be associated with susceptibility to preeclampsia.

Keywords: Single-nucleotide polymorphisms, Human leukocyte antigen-G, Un-translated Region, Preeclampsia